Cell diagnostics : images, biophysical and biochemical processes in allelopathy /

Topics discussed in this title include cellular model systems, new methods of microscopy in cellular diagnostics and methods of analytical biochemistry and biophysics.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Roshchina, V. V. (Viktorii͡a Vladimirovna), Narwal, Shamsher S.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Enfield, NH : Science Publishers, ©2007.
Temas:
Allelopathic agents > Research > Technique.
Allelopathy > Research > Technique.
Agents allélopathiques > Recherche > Technique.
Allélopathie > Recherche > Technique.
SCIENCE > Life Sciences > Anatomy & Physiology.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Foreword ; Preface ; Contents ; Detail of Contents ; List of Contributors ; Section 1-CELLULAR MODEL SYSTEMS ; 1. Allelopathy and Plant Cell Diagnostics; 1. Introduction; 2. Allelopathy; 3. Plant Cell Diagnostics; 4. References; 2. Cellular Models as Biosensors; 1. Introduction; 2. Cellular Biosensors and their Reactions; 2.1 Vegetative Microspores of Spore-Producing Plants; Principle:; Materials required:; Procedure:; Observations:; Statistical analysis:; Experiment 1.; Experiment 2.; Experiment 3.; 2.2 Pollens of Seed-Bearing Plants; Principle:; Materials required:; Procedure.
  • Observations:Statistical analysis:; Experiment 1; Experiment 2.; Experiment 3.; 2.3 Pistil Stigma (Female Gametophyte) of Seed-bearing Plants; Principle:; Materials required:; Procedure:; Observations:; Statistical analysis:; Experiment 1.; 3. Analysis of Allelochemical Binding in a Cell; 3.1 Hydrophilic Compounds ; Principle:; Materials required:; Procedure:; Observations:; Statistical analysis:; Experiment 1.; Experiment 2; 3.2 Hydrophobic Compounds (Fluorescence of Allelochemicals in Cell) ; Principle:; Materials required:; Procedure:; Observation:; Experiment 3.; 4. SUGGESTED READINGS.
  • 3. Microalgae for Determing the Effects of Allelochemicals1. Introduction; 2. Field Incubation Studies; 2.1 Field Incubation Procedure and Experiments; 3. Laboratrory Studies; 3.1 Continuous Culture Systems for Macrophytes and Microalgae; 3.2 Continuous Culture Methods for Microalgae; I. Turbidostat:; II. Chemostat:; 3.2.1 Continuous trapping apparatus ; 3.3 Algal Assays for Monitoring Alellochemicals Toxicity; 3.3.1 Two-step approach ; I. Filter paper disk bioassays; II. Bioassay in a liquid medium:; III. End Point test:; 4. Measurement of Response ; 4.1 Response Patterns.
  • 4.2 Effects of Allelochemicals on Algae4.2.1 Regreening of red colour cells of Chlorella zofingensis ; Advantages; 4.2.2 SCGE technique to assess genetic toxicity of allelochemicals ; 5. Mathematical Models; 6. References; 4. Bryophytes to Test Allelochemicals in vitro; 1. Introduction; (i). Chloronema:; (ii). Caulonema:; (iii). Rhizonema:; 2. Test of Co-Existence in vitro; 2.1 Materials Required; (i). Mohr medium:; (ii). Plant material:; (iii). Protonemal system from spores:; (iv). Protonemal system from shoots:; (v). Protonema regeneration from wild moss and liverwort:; 2.2 Procedure.
  • 2.3 Observations and Data Analysis3. Guided Isolation of Potential Allelochemicals; 3.1 Materials Required; Test fractions/extracts:; 3.2 Procedure; 3.3 Disadvantages; 4. Conclusions; 5. References; Section 2-NEW METHODS OF MICROSCOPY IN CELLULAR DIAGNOSTICS; 5. Microscopic Methods to Study Morpho-cytological Events during the Seed Germination; 1. Introduction; 2. Preparation of Rue Extract; 2.1 Radish (Raphanus Sativus); 2.1.1 Morphological and cytological responses. ; 2.1.1.1 Water uptake by radish seeds ; Experiment 1.; Procedure:; 2.1.1.2 Germination of radish seeds ; Experiment 1.

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