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Handbook of Vascular Biology Techniques
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are col...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor Corporativo: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
2015.
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| Edición: | 1st ed. 2015. |
| Temas: | |
| Acceso en línea: | Texto Completo |
Tabla de Contenidos:
- Preface
- Section 1 In vitro techniques
- In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation
- Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis
- Induction of hypoxia in vascular endothelial cell culture
- Evaluating In Vitro Angiogenesis using Live Cell Imaging
- Isolation of Endothelial Progenitor Cells (EPCs)
- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis
- Assessment of vascular function and contractility, ex vivo
- Measurement of Aβ uptake by cerebrovascular smooth muscle cells
- Measurement of Intracellular Ca2+ in Human Endothelial cells
- Section 2 In vivo and ex vivo manipulations
- Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion
- Sponge implant model of inflammatory angiogenesis
- The chick embryo chorioallantoic membrane assay
- Dorsal air sac assay
- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals
- Hypoxia-induced retinal angiogenesis in adult zebrafish
- Angiogenesis in regenerating adult zebrafish tail fin
- Methods for studying developmental angiogenesis in zebrafish
- Isolation and Expansion of Brain Microvascular Endothelial Cells
- Section 3 Imaging and histological analysis
- Adipose angiogenesis
- Assessing tumor angiogenesis in histological samples
- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea
- Computed Tomography Angiography: Fundamental techniques and data interpretation
- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation
- Single-Photon Emission Tomography of the brain in vascular pathology
- Section 4 Miscellaneous novel techniques in vascular biology
- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues
- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo
- Vascular Flow Modelling using Computational Fluid Dynamics
- Reverse Transcription Real-Time PCR Protocol for gene expression analyses
- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis
- Genomic microarray analysis
- Selection of appropriate housekeeping genes for quality control
- Endothelial transcriptomic analysis
- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers
- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification
- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood
- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry.