MicroRNA Expression Detection Methods

This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression, their application in miRNA research and their potential as tools for clinical diagnosis and prognosis. It begins with the introduction of the overall concept and strategies of...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autores principales: Wang, Zhiguo (Autor), Yang, Baofeng (Autor)
Autor Corporativo: SpringerLink (Online service)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2010.
Edición:1st ed. 2010.
Temas:
Cytology.
Biochemistry.
Molecular genetics.
Pharmacology.
Medical genetics.
Cell Biology.
Molecular Genetics.
Clinical Genetics.
Acceso en línea:Texto Completo
Tabla de Contenidos:
  • General Remarks
  • Detection, Profiling, and Quantification of miRNA Expression
  • miRNA Microarray Methods
  • Microarray and Its Variants for miRNA Profiling
  • Northern Blotting Methods
  • Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs
  • In Situ Hybridization Methods
  • In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs
  • Real-Time RT-PCR Methods
  • End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification
  • miR-Q RT-PCR for miRNA Quantification
  • Poly(A)-Tailed Universal Reverse Transcription
  • Multiplexing RT-PCR for High-Throughput miRNA Profiling
  • miRNA Amplification Profiling (mRAP)
  • Cloning Methods
  • miRNA Serial Analysis of Gene Expression (miRAGE or SAGE)
  • Nanoparticle Methods
  • Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis
  • Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling
  • Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis
  • Gold Nanoparticle Probe Method for miRNA Quantification
  • Other Methods
  • Splinted Ligation Method
  • Padlock-Probes and Rolling-Circle Amplification
  • Invader Assay
  • Single Molecule Method
  • Enzymatic Method
  • Surface-Enhanced Raman Spectroscopy Method
  • RAKE Assay
  • Bead-Based Flow Cytometric miRNA Expression Profiling
  • Bioluminescence miRNA Detection Method
  • Molecular Beacon Method
  • Ribozyme Method
  • Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis
  • Circulating miRNA Detection Methods
  • Serum and Plasma miRNA Detection
  • miRNA Detection from Peripheral Blood Microvesicles
  • Detection of Placental miRNAs in Maternal Plasma
  • Single-cell miRNA Detection Methods
  • Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell
  • Single Cell Stem-Looped Real-Time PCR
  • miRNA Function-Reporter Expression Assay
  • Whole Mount In Situ Analysis
  • Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.

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