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Protein Engineering

Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineeri...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Koehrer, Caroline (Editor ), RajBhandary, Uttam L. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2009.
Edición:1st ed. 2009.
Colección:Nucleic Acids and Molecular Biology, 22
Temas:
Acceso en línea:Texto Completo
Tabla de Contenidos:
  • Understanding Enzyme Mechanism through Protein Chimeragenesis
  • Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins
  • Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes
  • Chemical Methods for Mimicking Post-Translational Modifications
  • Noncanonical Amino Acids in Protein Science and Engineering
  • Fidelity Mechanisms of the Aminoacyl-tRNA Synthetases
  • Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes
  • In Vivo Studies of Receptors and Ion Channels with Unnatural Amino Acids
  • Synthesis of Modified Proteins Using Misacylated tRNAs
  • Cell-Free Synthesis of Proteins with Unnatural Amino Acids. The PURE System and Expansion of the Genetic Code
  • Engineering Nucleobases and Polymerases for an Expanded Genetic Alphabet
  • Understanding Membrane Proteins. How to Design Inhibitors of Transmembrane Protein-Protein Interactions.