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Nanoscale Imaging and Characterisation of Amyloid-β
This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting i...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Autor Corporativo: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cham :
Springer International Publishing : Imprint: Springer,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Springer Theses, Recognizing Outstanding Ph.D. Research,
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| Temas: | |
| Acceso en línea: | Texto Completo |
MARC
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| 001 | 978-3-319-39534-0 | ||
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| 005 | 20220120204555.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 160705s2016 sz | s |||| 0|eng d | ||
| 020 | |a 9783319395340 |9 978-3-319-39534-0 | ||
| 024 | 7 | |a 10.1007/978-3-319-39534-0 |2 doi | |
| 050 | 4 | |a QD95-96 | |
| 072 | 7 | |a PNFS |2 bicssc | |
| 072 | 7 | |a SCI078000 |2 bisacsh | |
| 072 | 7 | |a PNFS |2 thema | |
| 082 | 0 | 4 | |a 543 |2 23 |
| 100 | 1 | |a Tinker-Mill, Claire Louisa. |e author. |4 aut |4 http://id.loc.gov/vocabulary/relators/aut | |
| 245 | 1 | 0 | |a Nanoscale Imaging and Characterisation of Amyloid-β |h [electronic resource] / |c by Claire Louisa Tinker-Mill. |
| 250 | |a 1st ed. 2016. | ||
| 264 | 1 | |a Cham : |b Springer International Publishing : |b Imprint: Springer, |c 2016. | |
| 300 | |a XX, 149 p. 59 illus., 14 illus. in color. |b online resource. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Springer Theses, Recognizing Outstanding Ph.D. Research, |x 2190-5061 | |
| 505 | 0 | |a Introduction -- Literature Review and Theoretical Concepts -- Experimental Methodology -- Substrate Development of the Imaging of Amyloid Proteins with SPM Methods -- Scanning Probe Microscopy Methods of Imaging Amyloid Peptides During the Aggregation Process -- Spectroscopy and Thermal SPM Methods of Studying Aβ1:42 -- The Application of Biophysical Techniques to the Study of the Inhibition of Aggregation of Aβ Using PINPs Liposomes -- Conclusion and Future Perspectives. | |
| 520 | |a This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties. Amyloid-β (Aβ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Aβ with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes. | ||
| 650 | 0 | |a Spectrum analysis. | |
| 650 | 0 | |a Soft condensed matter. | |
| 650 | 0 | |a Medicine-Research. | |
| 650 | 0 | |a Biology-Research. | |
| 650 | 0 | |a Neurology . | |
| 650 | 0 | |a Nanotechnology. | |
| 650 | 1 | 4 | |a Spectroscopy. |
| 650 | 2 | 4 | |a Soft and Granular Matter. |
| 650 | 2 | 4 | |a Biomedical Research. |
| 650 | 2 | 4 | |a Neurology. |
| 650 | 2 | 4 | |a Nanotechnology. |
| 710 | 2 | |a SpringerLink (Online service) | |
| 773 | 0 | |t Springer Nature eBook | |
| 776 | 0 | 8 | |i Printed edition: |z 9783319395333 |
| 776 | 0 | 8 | |i Printed edition: |z 9783319395357 |
| 776 | 0 | 8 | |i Printed edition: |z 9783319819075 |
| 830 | 0 | |a Springer Theses, Recognizing Outstanding Ph.D. Research, |x 2190-5061 | |
| 856 | 4 | 0 | |u https://doi.uam.elogim.com/10.1007/978-3-319-39534-0 |z Texto Completo |
| 912 | |a ZDB-2-PHA | ||
| 912 | |a ZDB-2-SXP | ||
| 950 | |a Physics and Astronomy (SpringerNature-11651) | ||
| 950 | |a Physics and Astronomy (R0) (SpringerNature-43715) | ||