Plant Microtechniques and Protocols

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various struc...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Yeung, Edward Chee Tak (Editor ), Stasolla, Claudio (Editor ), Sumner, Michael John (Editor ), Huang, Bing Quan (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cham : Springer International Publishing : Imprint: Springer, 2015.
Edición:1st ed. 2015.
Temas:
Plant biotechnology.
Plants-Development.
Plants-Evolution.
Plant Biotechnology.
Plant Development.
Plant Evolution.
Acceso en línea:Texto Completo
Tabla de Contenidos:
  • A Guide to the Study of Plant Structure with Emphasis on Living Specimens
  • Chemical and Physical Fixation of Cells and Tissues: an Overview
  • Paraffin and Polyester Waxes
  • The Glycol Methacrylate Embedding Resins - Technovit 7100 and 8100
  • Epoxy Resins for Light and Transmission Electron Microscopy
  • LR White Acrylic Resin
  • High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples
  • Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens
  • Fluorescent Staining of Living Plant Cells
  • Improved Methods for Clearing and Staining of Plant Samples
  • Whole Mount Immunofluorescence Staining of Plant Cells and Tissues
  • Protoplast Isolation and Staining
  • Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving
  • Abscisic Acid Immunostaining
  • Plant Chromosome Preparations and Staining for Light Microscopic Studies
  • Chromosome techniques and FISH
  • Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2'-deoxyuridine (EdU)
  • Staining Methods for Programmed Cell Death
  • Laser Microdissection of Plant Tissues
  • RNA In situ Hybridization
  • Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy
  • Collecting and Processing Wood Microcores for Monitoring Xylogenesis
  • Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy
  • Collection of Plant Remains from Archaeological Contexts
  • Archaeological Wood Preparation
  • Archaeopalynological Preparation Techniques
  • Phytoliths: Preparation and Archaeological Extraction
  • Starch Granules: Preparation and Archaeological Extraction
  • Plant Collection, Identification, and Herbarium Procedures
  • Index.

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