Fluorescent Methods for Molecular Motors

This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Toseland, Christopher P. (Editor ), Fili, Natalia (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Basel : Springer Basel : Imprint: Springer, 2014.
Edición:1st ed. 2014.
Colección:Experientia Supplementum, 105
Temas:
Proteins .
Cytology.
Biophysics.
Protein Biochemistry.
Cell Biology.
Acceso en línea:Texto Completo
Tabla de Contenidos:
  • Fluorescence and Labelling: How to choose and what to do
  • Fluorescent biosensors: design and application to motor proteins
  • Rapid Reaction Kinetic Techniques
  • Fluorescence to study the ATPase mechanism of Motor Proteins
  • Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies
  • Fluorescent methods to study transcription initiation and transition into elongation
  • Single-molecule and single-particle imaging of molecular motors in vitro and in vivo
  • Fluorescence methods in the investigation of the DEAD-box helicase mechanism
  • Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins
  • Fluorescence Tracking of Motor proteins in vitro
  • Measuring Transport of Motor Cargos
  • Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET
  • Using fluorescence to study actomyosin in yeasts.

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