Lotus japonicus Handbook

Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by la...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Márquez, Antonio J. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Dordrecht : Springer Netherlands : Imprint: Springer, 2005.
Edición:1st ed. 2005.
Temas:
Botany.
Plant genetics.
Microbiology.
Botanical chemistry.
Agriculture.
Plant physiology.
Plant Science.
Plant Genetics.
Plant Biochemistry.
Plant Physiology.
Acceso en línea:Texto Completo
Tabla de Contenidos:
  • Lotus japonicus's a model system
  • Lotus-related species and their agronomic importance
  • Drought and saline stress
  • Methods for studying nodule development and function
  • A procedure for in vitro nodulation studies
  • Arbuscular mycorrhiza
  • Concurrent visualization of gusA and lacZ reporter gene expression
  • Embedding root and nodule tissue in plastic (BMM)
  • RNA isolation using phase extraction and LiCl precipitation
  • RNA isolation using CsCl gradients
  • 96-Well DNA isolation method
  • Genome structure analysis
  • Transcriptome analysis using cDNA arrays
  • Lotus japonicus expression database
  • Procedures for mass spectrometric proteome analysis
  • Isolation of peribacteroid membranes for proteome analysis
  • Metabolome analysis using GC-MS
  • Mutagenesis
  • A mutant catalogue of Lotus japonicus
  • Tilling
  • Ds gene-tagging
  • Mapping and map-based cloning
  • An in silico strategy towards the development of legume genome anchor markers using comparative sequence analysis
  • Information transfer: mapping and cloning in other legumes
  • Agrobacterium-mediated in vitro transformation
  • Induction of hairy roots for symbiotic gene expression studies
  • Transformation-regeneration procedure for Lotus japonicus
  • Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation
  • Vectors for reverse genetics and expression analysis
  • Nitrate assimilation: Influence of nitrogen supply
  • Nitrate and ammonium assimilatory enzymes
  • Transgenic plants affected in nitrate assimilation
  • Secondary metabolite profiling
  • Phenolic Compounds: extraction and analysis
  • Elicitation of isoflavan phytoalexins
  • An update of work published on Lotus japonicus.

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