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Microbial physiology and biochemistry laboratory : a quantitative approach /

Microbial Physiology and Biochemistry Laboratory illustrates the major features of growth and metabolism discussed in David White's The Physiology and Biochemistry of Prokaryotes (OUP, 1995). It serves as an ideal adjunct to this text and can also be used in conjunction with other books for the...

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Detalles Bibliográficos
Clasificación:QR88 W4.471
Autor principal: White, David, 1936- (autor)
Otros Autores: Hegeman, George (autor)
Formato: Libro
Idioma:Inglés
Publicado: New York : Oxford University Press, [1998].
©1998.
Temas:
Tabla de Contenidos:
  • Introduction.
  • Experiment 1. Exercise in pipetting and the Beer-Lambert Law.
  • Experiment 2. Bacterial Growth curve.
  • Experiment 3. Bioassay for Nicotine Acid.
  • Experiment 4. Effect of Environment on growth.
  • Experiment 5. Lactic Acid Production by Lactic Acid Bacteria.
  • Experiment 6. Assay of protein and RNA in whole cells grown at different growth rates.
  • Experiment 7. Analysis of Diauxic growth.
  • Experiment 8. Assay of Amylase and protease secreted by bacilus subtilis.
  • Experiment 9. Concentration of Amylase from Bacilus subtilis by Ammonium sulfate precipitation and separation from protease by affinity purification. Experiment 10. Ion-exchange chromatography of Amylase.