Cargando…
Microbial physiology and biochemistry laboratory : a quantitative approach /
Microbial Physiology and Biochemistry Laboratory illustrates the major features of growth and metabolism discussed in David White's The Physiology and Biochemistry of Prokaryotes (OUP, 1995). It serves as an ideal adjunct to this text and can also be used in conjunction with other books for the...
| Clasificación: | QR88 W4.471 |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Libro |
| Idioma: | Inglés |
| Publicado: |
New York :
Oxford University Press,
[1998].
©1998. |
| Temas: |
MARC
| LEADER | 00000aam a2200000 a 4500 | ||
|---|---|---|---|
| 001 | 000122267 | ||
| 005 | 20240202180208.2 | ||
| 007 | ta | ||
| 008 | 090207t19981998nyua g 000 0 eng d | ||
| 020 | |a 9780195113136 |q (papel libre de ácido) | ||
| 020 | |a 0195113136 |q (papel libre de ácido) | ||
| 040 | |a DLC |b spa |e rda |c DLC |d UKM |d OCLC |d MX-MxUAM | ||
| 041 | 0 | |a eng | |
| 050 | 4 | |a QR88 |b W4.471 | |
| 082 | 0 | 4 | |a 571.2/93 |
| 090 | |a QR88 |b W4.471 | ||
| 100 | 1 | |a White, David, |d 1936-, |e autor | |
| 245 | 1 | 0 | |a Microbial physiology and biochemistry laboratory : |b a quantitative approach / |c David White and George D. Hegeman. |
| 264 | 1 | |a New York : |b Oxford University Press, |c [1998]. | |
| 264 | 1 | |a ©1998. | |
| 300 | |a xi, 161 páginas : |b ilustraciones, gráficas en blanco y negro ; |c 28 cm. | ||
| 336 | |a texto |b txt |2 rdacontent | ||
| 337 | |a sin medio |b n |2 rdamedia | ||
| 338 | |a volumen |b nc |2 rdacarrier | ||
| 504 | |a Incluye referencias bibliográficas al final de cada experimento. | ||
| 505 | 2 | 0 | |t Introduction.-- |g Experiment 1. |t Exercise in pipetting and the Beer-Lambert Law. -- |g Experiment 2. |t Bacterial Growth curve.-- |g Experiment 3. |t Bioassay for Nicotine Acid.-- |g Experiment 4. |t Effect of Environment on growth.-- |g Experiment 5. |t Lactic Acid Production by Lactic Acid Bacteria.-- |g Experiment 6. |t Assay of protein and RNA in whole cells grown at different growth rates.-- |g Experiment 7. |t Analysis of Diauxic growth.-- |g Experiment 8. |t Assay of Amylase and protease secreted by bacilus subtilis.-- |g Experiment 9. |t Concentration of Amylase from Bacilus subtilis by Ammonium sulfate precipitation and separation from protease by affinity purification. |g Experiment 10. |t Ion-exchange chromatography of Amylase. |
| 520 | 1 | |a Microbial Physiology and Biochemistry Laboratory illustrates the major features of growth and metabolism discussed in David White's The Physiology and Biochemistry of Prokaryotes (OUP, 1995). It serves as an ideal adjunct to this text and can also be used in conjunction with other books for the laboratory component of a microbial physiology course. All of the experiments described in this manual have been taught as part of a laboratory course for junior and senior biology and microbiology majors at Indiana University. In addition to reinforcing what students learn in lecture, the experiments guide students through a wide spectrum of analytical techniques including enzyme assays, macromolecular assays, column chromatography, gel electrophoresis, and gas chromatography. Along with enzyme assays and enzyme purification, students do experiments measuring oxygen uptake, chemotaxis, fermentation, and bacterial luminescence. The organisms studied include Escherichia, Pseudomonas, Bacillus, Proteus, Rhodospirillum, Photobacterium, and Saccharomyces. The volume is enhanced by appendices which include sections on quantitative problems and their solutions, instructions on how to write a laboratory report, and independent projects that are extensions of the class experiments. The number of experiments exceeds the amount of material usually offered in one semester, giving instructors the option to choose those experiments that are most appropriate for their classes. | |
| 538 | |a Comunidad/CBS/Presupuesto Biblioteca 156.01.01.92/ICL20070100/IBI20070122/COBI. Fac-14982/[w249148/$507.00] | ||
| 650 | 0 | |a Microbial metabolism |v Laboratory manuals | |
| 650 | 4 | |a Metabolismo microbiano |v Manuales de laboratorio | |
| 650 | 0 | |a Bacteria |x Physiology |v Laboratory manuals | |
| 650 | 4 | |a Bacterias |x Fisiología |v Manuales de laboratorio | |
| 772 | 0 | |i Suplemento de: |a White, David, 1936-. |t Physiology and biochemistry of prokaryotes. |b 3rd edition. |d New York : Oxford University, 2007. |w 120664 | |
| 700 | 1 | |a Hegeman, George, |e autor | |
| 905 | |a LIBROS | ||
| 938 | |a Comunidad |c CBS |d Presupuesto Biblioteca 156.01.01.92 |e ICL20070100 |f IBI20070122 | ||
| 949 | |a Biblioteca UAM Iztapalapa |b Colección General |c QR88 W4.471 | ||